北海道大学農学部 植物病原学研究室

講師　中原健二

農学部総合研究棟W306
knakahar@agr.hokudai.ac.jp

講師　畑谷達児

農学部総合研究棟W307
hataya@agr.hokudai.ac.jp

2017.3.23 卒業式

• ウイルスと植物との闘いの中で、病気がどのように起きるのか知りたい！
• ウイルス感染でトマトのビタミンCが上がり､トウガラシが辛くなるのはなぜか?
• ウイルスに罹らない抵抗性作物を育種するための新たな技術を開発しよう
• 小さ過ぎて簡単には見えないウイルスを診断する方法を確立しよう
• 植物ウイルスでヒトの病気のワクチンを作ろう

研究材料の植物

タバコ トマト トウガラシ ニンニク シロイヌナズナ　アスパラガス　イチゴ　ジャガイモ　ダイズ　エンドウ　ソラマメ

研究トピック2:

研究トピック1(中原): ウイルスの強毒化を防ぐエンドウの仕組みを発見

ウイルスはその高い増殖力や変異頻度のため, 薬剤耐性を獲得したり, 動植物の免疫機構を回避したりして病気を引き起こす強毒ウイルスに進化してしまう。このことがウイルス病の制御を困難にする。これに対抗して, エンドウは別々の免疫システム二つを協働させて, ウイルスが一方の免疫システムを回避するよう進化するともう一方の免疫システムにその感染がより強く阻害されるように仕組まれていた。このウイルスにトレードオフ（痛し痒し）を強いることで強毒ウイルスへの進化を防ぐこと（図参照）を厚見　剛　研究員（現, 国立研究開発法人産業技術総合研究所）, 中原　健二植物病原学研究室 講師らの研究グループが発見した。進化が早いウイルスに対抗するための植物の防御戦略であると思われる。本知見を活用することで, 将来, ウイルス病を発症しない性質=抵抗性が永続する作物を育種するための新たな抵抗性育種技術の開発が期待される。本成果は2016年6月8日に米国微生物学会誌Journal of Virology (http://dx.doi.org/10.1128/JVI.00190-16)に発表された。また, 関連の総説が6月13日に米国植物病理学会誌Molecular Plant-Microbe Interactions (http://dx.doi.org/10.1094/MPMI-05-16-0103-CR)に発表された。

図1 ウイルスにトレードオフを強いることで強毒化を防ぐ植物の仕組みを比喩的に表した変則ジャンケン.

ウイルスは後出しする権利(進化が速い)を持つが, その代わり植物は一度に二つの手を出す権利(同じウイルスに対する二つの免疫機構)を持つ. この時, ウイルスは引き分けに持ち込む(強毒化しない)しかない.

１．公務員：農水省　北海道庁　青森県試　栃木県試　埼玉県試　兵庫県試横浜植物防疫所　香川県庁　鳥取県産業技術センター

２．会社：セイコーマート　カゴメ　サカタのタネ　ホクレン　クミアイ化学　ノーステック財団（札幌）JA全農　吉田製薬　インテリジェンス　雪印種苗　JT日本たばこ

2021年

Jayasinghe, W.H., Akhter, Md.S., Nakahara, K., Maruthi, M.N. (2021) Effect of aphid biology and morphology on plant virus transmission. Pest Management Science online ahead print

Akhter, Md.S., Nakahara, K.S., Masuta, C. (2021) Resistance induction based on the understanding of molecular interactions between plant viruses and host plants. Virology Journal 18, 176

Naoi, T., Hataya, T. (2021) Tolerance even to lethal strain of potato spindle tuber viroid found in wild tomato species can be introduced by crossing. Plants 10, 575

Osmani, Z., Sabet, M.S., Nakahara, K.S., Mokhtassi-Bidgoli A., Vahabi, K., Moieni, A., Shams-Bakhsh, M. (2021) Identification of a defense response gene involved in signaling pathways against PVA and PVY in potato. GM Crops & Food 12, 86-105

2020年

Kobayashi, Y., Fukuzawa, N., Hyodo, A., Kim, H., Mashiyama, S., Ogihara, T., Yoshioka, H., Matsuura. H., Masuta, C., Matsumura, T., Takeshita, M. (2020) Role of salicylic acid glucosyltransferase in balancing growth and defence for optimum plant fitness. Molecular Plant Pathology 21, 429-442

Taninaka, Y., Nakahara, K.S., Yuka Hagiwara-Komoda, Y. (2020) Intracellular proliferation of clover yellow vein virus is unaffected by the recessive resistance gene cyv1 of Pisum sativum. Microbiology and Immunology 64, 76-82

Wang, Y., Xu, W., Abe, J., Nakahara, K.S., Hajimorad, M.R. (2020) Precise exchange of HC-Pro cistron between soybean mosaic virus and clover yellow vein virus: Impact on virus viability and host range specificity. Phytopathology 110, 206-214

2019年

Osmani, Z., Jin, S., Mikami, M., Endo, M., Atarashi, H., Fujino, K., Yamada, T., Nakahara, K.S. (2019) CRISPR/Cas9-mediated editing of genes encoding rgs-CaM-like proteins in transgenic potato plants. In: Kobayashi K., Nishiguchi M. (eds) Antiviral resistance in plants. Methods in Molecular Biology 2028, 153-165 Humana, New York, NY

Abe, J., Wang, Y., Yamada, T., Sato, M., Ono, T., Atsumi, G., Abe, J., Hajimorad, M.R., Nakahara, K.S. (2019) Recessive resistance governed by a major quantitative trait locus restricts clover yellow vein virus in mechanically but not graft-inoculated cultivated soybeans. Molecular Plant-Microbe interactions 32, 1026-1037

Kim, H., Shimura, H., Masuta, C. (2020) Advancing toward commercial application of RNA silencing‑based strategies to protect plants from viral diseases. Journal of General Plant Pathology 85, 321-328

Li, X., Hataya, T. (2019) Construction and characterization of an infectious cDNA clone of potato virus S developed from selected populations that survived genetic bottlenecks. Virology Journal 16, 18

Matsunaga, W., Shimura, H., Shirakawa, S., Isoda, R., Inukai, T., Matsumura, T., Masuta, C. (2019) Transcriptional silencing of 35S driven-transgene is differentially determined depending on promoter methylation heterogeneity at specific cytosines in both plus- and minus-sense strands. BMC Plant Biology 19, 24

2018年

Fukuzawa, N., Masuta, C., Matsumura, T. (2018). Rapid transient protein production by the coat protein-deficient cucumber mosaic virus vector: non-packaged CMV system, NoPaCS. Plant Cell Reports 37, 1513-1522

Shimura, H., Masuta, C., Koda, Y. (2018). Metagenomic Analyses of the Viruses Detected in Mycorrhizal Fungi and Their Host Orchid. Methods in Molecular Biology 1746, 161-172

Yoshida, N., Shimura, H., Masuta, C. (2018). Allexiviruses may have acquired inserted sequences between the CP and CRP genes to change the translation reinitiation strategy of CRP. Archives of Virology 163, 1419-1427

2017年

Jeon, E.J., Tadamura, K., Murakami, T., Inaba, J.I., Kim, B.M., Sato, M, Atsumi, G., Kuchitsu, K., Masuta, C., Nakahara, K.S. (2017). rgs-CaM detects and counteracts viral RNA silencing suppressors in plant immune priming. Journal of Virology 91, e00761-17

中原 健二. (2017). トレードオフを利用した植物のウイルス防御戦略. 生化学 89, 436-440

Furuta, K., Nagashima S., Inukai T., Masuta C. (2017). Construction of a system for the strawberry nursery production towards elimination of latent infection of Anthracnose fungi by a combination of PCR and microtube hybridization. Plant Pathology Journal 33, 80-86

Suzuki., T., Fujibayashi, M., Hataya, T., Taneda, A., He, Y.H., Tsushima, T., Duraisamy, G.S., Siglová, K., Matoušek, J., Sano, T. (2017). Characterization of host-dependent mutations of apple fruit crinkle viroid replicating in newly identified experimental hosts suggests maintenance of stem–loop structures in the left-hand half of the molecule is important for replication. Journal of General Virology 98, 506-516

Ito M., Meguro-Maoka A., Maika T., Akino, S., Masuta, C. (2017). Increased susceptibility of potato to Rhizoctonia diseases in Potato leafroll virus-infected plants. Journal of General Plant Pathology 83, 169-172

2016年

Murota, K., Shimura, H., Takeshita, M., Masuta, C. (2016). Interaction between Cucumber mosaic virus 2b protein and plant catalase induces a specific necrosis in association with proteasome activity. Plant Cell Reports 36, 37-47

Murakami, T., Tayama, R., Nakahara, K.S. (2016). Microperforated leaf blotting on polyvinylidene difluoride and nylon membranes to analyze spatial distribution of endogenous and viral gene expression in plant leaves. Journal of General Plant Pathology 82, 254-260

Miyashita, Y., Atsumi, G., Nakahara, K.S. (2016). Trade-offs for viruses in overcoming innate immunities in plants. Molecular Plant-Microbe Interactions 29, 595-598

Atsumi, G., Suzuki, H., Miyashita, Y., Choi, S.H., Hisa, Y., Rihei, S., Shimada, R., Jeon, E.J., Abe, J., Nakahara, K.S., Uyeda, I. (2016). P3N-PIPO, a frameshift product from P3, pleiotropically determines the virulence of clover yellow vein virus in both resistant and susceptible peas. Journal of Virology 90, 7388−7404

Fujiwara A., Togawa S., Hikawa T., Matsuura H., Masuta C., Inukai T. (2016). Ascorbic acid accumulates as a defense response to Turnip mosaic virus in resistant Brassica rapa cultivars. Journal of Experimental  Botany 67, 4391-4402

Kikuchi Y, Hijikata N, Ohtomo R, Handa Y, Kawaguchi M, Saito K, Masuta C, Ezawa T. (2016). Aquaporin-mediated long-distance polyphosphate translocation directed towards the host in arbuscular mycorrhizal symbiosis: application of virus-induced gene silencing. New Phytologist

Yamashita Y, Ota M, Inoue Y, Hasebe Y, Okamoto M, Inukai T, Masuta C, Sakihama Y, Hashidoko Y, Kojima M, Sakakibara H, Inage Y, Takahashi K, Yoshihara T, Matsuura H. (2016). Chemical promotion of endogenous amounts of ABA in Arabidopsis thaliana by a natural product, theobroxide. Plant & Cell Physiology 57, 986-999

Hagiwara-Komoda, Y., Choi, S.H., Sato, M., Atsumi, G., Abe, J., Fukuda, J., Honjo, M.N., Nagano, A.J., Komoda, K., Nakahara, K.S., Uyeda I., Naito S. (2016). Truncated yet functional viral protein produced via RNA polymerase slippage implies underestimated coding capacity of RNA viruses. Scientific Reports 6, 21411

Shimura, H., Masuta C. (2016). Plant subviral RNAs as a long noncoding RNA (lncRNA): Analogy with animal lncRNAs in host-virus interactions. Virus Research  212, 25-9

2015年

Wagh, S.G., Kobayashi, K., Yaeno, T., Yamaoka, N., Masuta, C., Nishiguchi, M. (2015). Rice necrosis mosaic virus, a fungal transmitted Bymovirus: complete nucleotide sequence of the genomic RNAs and subgrouping of bymoviruses. Journal of General Plant Pathology 82, 38-42

Yamagishi, M., Masuta, C., Suzuki, M., Netsu, O. (2015). Peanut stunt virus-induced gene silencing in white lupin (Lupinus albus). Plant Biotechnology 32,181-191

Nakahara, K.S., Nishino, K., Uyeda, I. (2015). Construction of infectious cDNA clones derived from the potyviruses Clover yellow vein virus and Bean yellow mosaic virus. Methods in Molecular Biology 1236, 219-227

2014年

忠村一毅, 中原健二 (2014). ウイルスに対する植物の自然免疫機構. 化学と生物, 52 (12), 805-813

中原健二, 忠村一毅, Eun Jin Jeon (2014). タバコの病害抵抗性におけるカルモジュリン様タンパクrgs-CaMの機能と役割. 植物感染生理談話会論文集, 第49号 59-68頁, 新視点から見渡す病原体感染戦略と植物免疫ネットワーク

Nakahara, K.S., Masuta, C. (2014). Interaction between viral RNA silencing suppressors and host factors in plant immunity. Current Opinion in Plant Biology 20, 88-95

Hisa, Y., Suzuki, H., Atsumi, G., Choi, S.H., Nakahara, K.S., Uyeda, I. (2014). P3N-PIPO of Clover yellow vein virusexacerbates symptoms in pea infected with White clover mosaic virus and is implicated in viral synergism. Virology 449, 200-206

2013年

Choi, S.H., Hagiwara-Komoda, Y., Nakahara, K.S., Atsumi, G., Shimada, R., Hisa, Y., Naito, S., Uyeda, I. (2013). Quantitative and qualitative involvement of P3N-PIPO in overcoming recessive resistance against Clover yellow vein virus in pea carrying cyv1. Journal of Virology 87, 7326-7337

Maoka, T., Nakayama, T., Taniguchi, M., Kano, Y., Suzuki, A., Sato, M., Hataya, T., Koizumi, E., Noguchi, K. (2013). Multivirus detection from Japanese landraces of potato by reverse transcription–polymerase chain reaction–microplate hybridization. Potato Research 56, 147−156

2012年

Ogawa, T., Nakagawa, A., Hataya, T., Ohshima, K. (2012). The genetic structure of populations of Potato virus Y in Japan; based on the analysis of 20 full genomic sequences. Journal of Phytopathology 160, 661-673

Tadamura, K., Nakahara, K.S., Masuta, C., Uyeda, I. (2012). Wound-induced rgs-CaM gets ready for counterresponse to an early stage of viral infection. Plant Signaling & Behavior 12, 1548-1551

Nakahara, K.S., Masuta, C., Yamada, S., Shimura, H., Kashihara, Y., Wada, T.S., Meguro, A., Goto, K., Tadamura, K., Sueda, K., Sekiguchi, T., Shao, J., Itchoda, N., Matsumura, T., Igarashi, M., Ito, K., Carthew, R.W., Uyeda, I. (2012). Tobacco calmodulin-like protein provides secondary defense by binding to and directing degradation of virus RNA silencing suppressors. Proceedings of the National Academy of Sciences USA 109, 10113-10118

Choi, S.H., Nakahara, K.S., Andrade, M., Uyeda, I. (2012). Characterization of the recessive resistance gene cyv1 against Clover yellow vein virus in Pisum sativum. Journal of General Plant Pathology 78, 269-276

Atsumi, G., Nakahara, K.S., Wada, T.S., Choi, S.H., Masuta, C., Uyeda, I. (2012). Heterologous expression of viral suppressors of RNA silencing complements virulence of the HC-Pro mutant of clover yellow vein virus in pea. Archives of Virology 157, 1019-1028

Ido, Y., Nakahara, K.S., Uyeda, I. (2012). White clover mosaic virus-induced gene silencing in pea. Journal of General Plant Pathology 78,  127-132

2011年

Nakahara, K.S., Kitazawa, H., Atsumi, G., Choi, S.H., Suzuki, Y., Uyeda, I. (2011). Screening and analysis of genes expressed upon infection of broad bean with Clover yellow vein virus causing lethal necrosis. Virology Journal 8, 355.

Nakahara, K.S., Yoshida, K., Suzaki, K., Yoshikawa, N. and Ito, T. (2011). Sensitive PCR-based detection of Apple chlorotic leaf spot virus heterogenous in apple trees. Japan Agricultural Research Quarterly 45, 411-421.

Harris, D.A., Kim, K., Nakahara, K., Vasquez-Doorman, C., and Carthew, R.W. (2011). Cargo sorting to lysosome-related organelles regulates siRNA-mediated gene silencing. Journal of Cell Biology 194, 77-87.

2010年

Maoka, T., Sugiyama, S., Maruta, Y., Hataya, T. (2010). Application of cDNA Macroarray for Simultaneous Detection of 12 Potato Viruses. Plant Disease 94, 1248-1254.

Nakayama, T., Maoka, T., Hataya, T., Shimizu, M., Fuwa, H., Tsuda, S., and Mori, M. (2010). Diagnosis of Potato Mop-Top Virus in Soil Using Bait Plant Bioassay and RT-PCR-microplate Hybridization. American Journal of Potato Research 87, 218-225.

Uyeda, I. (2010). What can we learn from basic plant virology? Journal of General Plant Pathology 76, 399-402.

Nakahara, K.S., Shimada, R., Choi, S.H., Yamamoto, H., Shao, J., and Uyeda, I. (2010). Involvement of the P1 cistron in overcoming eIF4E-mediated recessive resistance against Clover yellow vein virus in pea. Molecular Plant-Microbe Interactions 23, 1460-1469.

2009年

Hataya, T. (2009). Duplex reverse transcription-polymerase chain reaction system to detect Potato spindle tuber viroid using an internal control mRNA and a non-infectious positive control RNA. Journal of General Plant Pathology 75, 167-172.

Atsumi, G., Kagaya, U., Kitazawa, H., Nakahara, K.S. and Uyeda, I. (2009). Activation of the salicylic acid signaling pathway enhances Clover yellow vein virus virulence in susceptible pea cultivars. Molecular Plant-Microbe Interactions 22, 166-175.

Andrade, M., Abe, Y., Nakahara, K.S. and Uyeda, I. (2009). The cyv-2 resistance to Clover yellow vein virus in pea is controlled by the eukaryotic initiation factor 4E. Journal of General Plant Pathology 75, 241-249.

Lee, Y.S., Pressman, S., Andress, A.P., Kim, K., White, J.L., Cassidy, J.J., Li, X., Lubell, K., Lim, D.H., Cho, I.S., Nakahara, K., Preall, J.B., Bellare, P., Sontheimer, E.J., and Carthew, R.W. (2009). Silencing by small RNAs is linked to endosomal trafficking. Nature Cell Biology 11, 1495-1495.

2008年

Yambao, M.L.M., Yagihashi, H., Sekiguchi, H., Sekiguchi, T., Sasaki, T., Sato, M., Atsumi, G., Tacahashi, Y., Nakahara, K.S. and Uyeda, I. (2008). Point mutations in helper component protease of clover yellow vein virus are associated with the attenuation of RNA-silencing suppression activity and symptom expression in broad bean. Archives of Virology 153, 105-115.

2007年

Ravelo, G., Kagaya, U., Inukai, T., Masanao Sato · Ichiro Uyeda, I. (2007). Genetic analysis of lethal tip necrosis induced by Clover yellow vein virus infection in pea. Journal of General Plant Pathology 73, 59–65.

Andrade, M., Sato, M., and Uyeda, I. (2007). Two resistance modes to Clover yellow vein virus in pea characterized by a green fluorescent protein-tagged virus. Phytopathology 97, 544-550.

中原健二, 厚見剛, 上田一郎 (2007). クローバー葉脈黄化ウイルスの病原性. 植物感染生理談話会論文集, 第43号, 植物-病原微生物の相互作用のダイナミズム

2006年

Kim, K., Lee, Y.S., Harris, D., Nakahara, K., and R.W. Carthew (2006). The RNAi Pathway Initiated by Dicer-2 in Drosophila. Cold Spring Harbor Symposia on Quantitative Biology 71, 39-44.

2005年

Maoka, T., and Hataya, T. (2005). The complete nucleotide sequence and biotype variability of Papaya leaf distortion mosaic virus. Phytopathology 95, 128-135.

Nakahara, K., Kim, K., Sciulli, C., Dowd, S.R., Minden, J.S., and Carthew, R.W. (2005). Targets of microRNA regulation in the Drosophila oocyte proteome. Proceedings of the National Academy of Sciences USA 102, 12023-12028.

Hatfield, S.D., Shcherbata, H.R., Fischer, K.A., Nakahara, K., Carthew, R.W., Ruohola-Baker, H. (2005). Stem cell division is regulated by the microRNA pathway. Nature 435, 974-978.

Sekiguchi, H., Isogai, M., Masuta, C., and Uyeda, I. (2005). 3C-like protease encoded by Rice tungro spherical virus is autocatalytically processed. Archives of virology 150, 595-601.

Sato, M., Nakahara, K., Yoshii, M., Ishikawa, M., and Uyeda, I. (2005). Selective involvement of members of the eukaryotic initiation factor 4E family in the infection of Arabidopsis thaliana by potyviruses. FEBS letters 579, 1167-1171.

Morita-Yamamuro, C., Tsutsui, T., Sato, M., Yoshioka, H., Tamaoki, M., Ogawa, D., Matsuura, H., Yoshihara, T., Ikeda, A., Uyeda, I., et al.(2005). The Arabidopsis gene CAD1 controls programmed cell death in the plant immune system and encodes a protein containing a MACPF domain. Plant & Cell Physiology 46, 902-912.

2004年

Kunii, M., Kanda, M., Nagano, H., Uyeda, I., Kishima, Y., and Sano, Y. (2004). Reconstruction of putative DNA virus from endogenous rice tungro bacilliform virus-like sequences in the rice genome: implications for integration and evolution. BMC Genomics 5, 80.

Nakahara, K., and Carthew, R.W. (2004). Expanding roles for miRNAs and siRNAs in cell regulation. Current Opinion in Cell Biology 16, 127-133.

Lee, Y.S., Nakahara, K., Pham, J.W., Kim, K., He, Z., Sontheimer, E.J., and Carthew, R.W. (2004) Distinct Roles for DrosophilaDicer-1 and Dicer-2 in the siRNA/miRNA Silencing Pathways. Cell 117, 69-81.

2003年

Yambao, M.L., Masuta, C., Nakahara, K., and Uyeda, I. (2003). The central and C-terminal domains of VPg of Clover yellow vein virus are important for VPg-HCPro and VPg-VPg interactions. Journal of General Virology 84, 2861-2869.

Sekiguchi, H., Tacahashi, Y., and Uyeda, I. (2003). The 3′ terminal region is strictly required for clover yellow vein virus genome replication. Archives of Virology 148, 759-772.

Sato, M., Masuta, C., and Uyeda, I. (2003). Natural resistance to Clover yellow vein virus in beans controlled by a single recessive locus. Molecular Plant-Microbe Interactions 16, 994-1002.

Hong, J.S., Masuta, C., Nakano, M., Abe, J., and Uyeda, I. (2003). Adaptation of Cucumber mosaic virus soybean strains (SSVs) to cultivated and wild soybeans. Theoretical and Applied Genetics 107, 49-53.

2002年

Ito, T., Ieki, H., Ozaki, K., Iwanami, T., Nakahara, K., Hataya, T., Ito, T., Isaka, M., and Kano, T. Multiple citrus viroids in citrus from Japan and their ability to produce exocortis-like symptoms in citron. Phytopathology 92, 542-547.

2001年

Hataya, T., Arimoto, R., Suda, N., and Uyeda, I. (2001). Molecular characterization of Hop mosaic virus: its serological and molecular relationships to Hop latent virus. Archives of Virology 146, 1935-1948.

Nakahara, K., Yoshida, K., Ito, T., Suzaki, K., and Kudo, A. (2001). Cloning and sequencing of endochitinase genes from Gliocladium virens and Trichoderma species. Archives of Phytopathology and Plant Protection 33, 519-527.

2000年

Uyeda, I. (2000). [Plant expression vectors based on plus-sense single-stranded RNA viruses]. Uirusu 50, 251-257.

Masuta, C., Yamana, T., Tacahashi, Y., Uyeda, I., Sato, M., Ueda, S., and Matsumura, T. (2000). Development of clover yellow vein virus as an efficient, stable gene-expression system for legume species. Plant J 23, 539-546.

Isogai, M., Cabauatan, P.Q., Masuta, C., Uyeda, I., and Azzam, O. (2000). Complete nucleotide sequence of the rice tungro spherical virus genome of the highly virulent strain Vt6. Virus Genes 20, 79-85.

Hataya, T., Uchino, K., Arimoto, R., Suda, N., Sano, T., Shikata, E., and Uyeda, I. (2000). Molecular characterization of Hop latent virus and phylogenetic relationships among viruses closely related to carlaviruses. Archives of Virology 145, 2503-2524.

1999年

Masuta, C., Nishimura, M., Morishita, H., Hataya, T. (1999). A single amino acid change in viral genome-associated protein of potato virus Y correlates with resistance breaking in ‘Virgin A Mutant’ tobacco. Phytopathology 89, 118-123.

Hataya, T., Nakahara, K., Furuta K., and Shikata, E. (1999). Comparisons of gene diagnostic methods for the practical diagnosis of chrysanthemum stunt viroid in chrysanthemum plants. Archives of Phytopathology and Plant Protection 32, 179-192.

Ueda, S., Masuta, C., and Uyeda, I. (1999). The C-terminal region of the P3 structural protein of rice dwarf phytoreovirus is important for P3-P3 interaction. Archives of Virology 144, 1653-1657.

Tacahashi, Y., and Uyeda, I. (1999). Restoration of the 3′ end of potyvirus RNA derived from Poly(A)-deficient infectious cDNA clones. Virology 265, 147-152.

Nakahara, K., Hataya, T., and Uyeda, I. (1999). A simple, rapid method of nucleic acid extraction without tissue homogenization for detecting viroids by hybridization and RT-PCR. Journal of Virological Methods 77, 47-58.

1998年

Hataya, T., Nakahara, K., Ohara, T., Ieki, H., Kano, T. (1998). Citrus viroid Ia is a derivative of citrus bent leaf viroid (CVd-Ib) by partial sequence duplications in the right terminal region. Archives of Virology 143, 971-980.

Uyeda, I., Lee, B.C., Ando, Y., Suga, H., He, Y.K., and Isogai, M. (1998). Reovirus isolation and RNA extraction. Methods in Molecular Biology (Clifton, NJ 81, 65-75.

Nakahara, K., Hataya, T., and Uyeda, I. (1998). Inosine 5′-triphosphate can dramatically increase the yield of NASBA products targeting GC-rich and intramolecular base-paired viroid RNA. Nucleic Acids Research 26, 1854-1856.

Nakahara, K., Hataya, T., Hayashi, Y., Sugimoto, T., Kimura, I., Shikata, E. (1998). A mixture of synthetic oligonucleotide probes labeled with biotin for the sensitive detection of potato spindle tuber viroid. Journal of Virological Methods 71, 219-227.

Nakaraha, K., Hataya, T., Uyeda I., and Ieki, H. (1998). An improved procedure for extracting nucleic acids from citrus tissues for diagnosis of citrus viroids. Annals of the Phytopathologicial Society of Japan 64, 532-538.

Masuta, C., Ueda, S., Suzuki, M., and Uyeda, I. (1998). Evolution of a quadripartite hybrid virus by interspecific exchange and recombination between replicase components of two related tripartite RNA viruses. Proceedings of the National Academy of Sciences USA 95, 10487-10492.

Isogai, M., Uyeda, I., and Lee, B.C. (1998). Detection and assignment of proteins encoded by rice black streaked dwarf fijivirus S7, S8, S9 and S10. Journal of General Virology 79, 1487-1494.

Isogai, M., Uyeda, I., and Lindsten, K. (1998). Taxonomic characteristics of fijiviruses based on nucleotide sequences of the oat sterile dwarf virus genome. Journal of General Virology 79, 1479-1485.

1997年

Ueda, S., Masuta, C., and Uyeda, I. (1997). Hypothesis on particle structure and assembly of rice dwarf phytoreovirus: interactions among multiple structural proteins. Journal of General Virology 78, 3135-3140.

Takahashi, Y., Takahashi, T., and Uyeda, I. (1997). A cDNA clone to clover yellow vein potyvirus genome is highly infectious. Virus Genes 14, 235-243.

1996年

Murao, K., Uyeda, I., Ando, Y., Kimura, I., Cabauatan, P.Q., and Koganezawa, H. (1996). Genomic rearrangement in genome segment 12 of rice dwarf phytoreovirus. Virology 216, 238-240.

1995年

Uyeda, I., Ando, Y., Murao, K., and Kimura, I. (1995). High resolution genome typing and genomic reassortment events of rice dwarf Phytoreovirus. Virology 212, 724-727.

Uyeda, I., Kimura, I., and Shikata, E. (1995). Characterization of genome structure and establishment of vector cell lines for plant reoviruses. Advances in Virus Research 45, 249-279.

Uyeda, I., Suga, H., Lee, S.Y., Yan, J., Hataya, T., Kimura, I., and Shikata, E. (1995). Rice ragged stunt Oryzavirus genome segment 9 encodes a 38 600 Mr structural protein. Journal of General Virology 76, 975-978.

Suga, H., Uyeda, I., Yan, J., Murao, K., Kimura, I., Tiongco, E.R., Cabautan, P., and Koganezawa, H. (1995). Heterogeneity of rice ragged stunt oryzavirus genome segment 9 and its segregation by insect vector transmission. Archives of Virology 140, 1503-1509.

1994年

Hataya T., Inoue, A.K., Shikata, E. (1994). A PCR-microplate hybridization method for plant-virus detection. Journal of Virological Mehtods 46, 223-236.

Hataya, T., Inoue, A.K., Ohshima, K., and Shikata, E. (1994). Characterization and strain identification of a potato-virus-Y isolate nonreactive with monoclonal-antibodies specific to the ordinary and necrotic strains. Intervirology 37, 12-19.

Uyeda, I., Suda, N., Yamada, N., Kudo, H., Murao, K., Suga, H., Kimura, I., Shikata, E., Kitagawa, Y., Kusano, T., et al. (1994). Nucleotide sequence of rice dwarf phytoreovirus genome segment 2: completion of sequence analyses of rice dwarf virus. Intervirology 37, 6-11.

Murao, K., Suda, N., Uyeda, I., Isogai, M., Suga, H., Yamada, N., Kimura, I., and Shikata, E. (1994). Genomic heterogeneity of rice dwarf phytoreovirus field isolates and nucleotide sequences of variants of genome segment 12. Journal of General Virology 75, 1843-1848.

1993年

Azuhata, F., Uyeda, I., Kimura, I., and Shikata, E. (1993). Close similarity between genome structures of rice black-streaked dwarf and maize rough dwarf viruses. Journal of General Virology 74, 1227-1232.

1992年

Ohshima, K., Nakaya, T., Inoue, A.K., Hataya, T., Hayashi, Y., and Shikata, E. (1992). Production and characteristics of strain common antibodies against a synthetic polypeptide corresponding to the C-terminal region of potato virus-Y coat protein. Journal of Virological Methods 40, 265-273.

Yan, J., Kudo, H., Uyeda, I., Lee, S.Y., and Shikata, E. (1992). Conserved terminal sequences of rice ragged stunt virus genomic RNA. Journal of General Virology 73, 785-789.

Uyeda, I. (1992). Bean yellow mosaic virus subgroup; search for the group specific sequences in the 3′ terminal region of the genome. Archives of Virology 5, 377-385.

Uyeda, I. (1992). [Genome structure of plant reoviruses]. Tanpakushitsu kakusan koso 37, 2462-2466.

Suzuki, N., Tanimura, M., Watanabe, Y., Kusano, T., Kitagawa, Y., Suda, N., Kudo, H., Uyeda, I., and Shikata, E. (1992). Molecular analysis of rice dwarf phytoreovirus segment S1: interviral homology of the putative RNA-dependent RNA polymerase between plant- and animal-infecting reoviruses. Virology 190, 240-247.

Azuhata, F., Uyeda, I., and Shikata, E. (1992). Conserved terminal nucleotide sequences in the genome of rice black streaked dwarf virus. Journal of General Virology 73, 1593-1595.

1991年

Uyeda, I., Takahashi, T., and Shikata, E. (1991). Relatedness of the nucleotide sequence of the 3′-terminal region of clover yellow vein potyvirus RNA to bean yellow mosaic potyvirus RNA. Intervirology 32, 234-245.

Kudo, H., Uyeda, I., and Shikata, E. (1991). Viruses in the phytoreovirus genus of the Reoviridae family have the same conserved terminal sequences. Journal of General Virology 72, 2857-2866.

1990年

Yamada, N., Uyeda, I., Kudo, H., and Shikata, E. (1990). Nucleotide sequence of rice dwarf virus genome segment 3. Nucleic Acids Research 18, 6419.

Uyeda, I., Kudo, H., Yamada, N., Matsumura, T., and Shikata, E. (1990). Nucleotide sequence of rice dwarf virus genome segment 4. Journal of General Virology 71, 2217-2222.

1989年

Uyeda, I., Kudo, H., Takahashi, T., Sano, T., Ohshima, K., Matsumura, T., and Shikata, E. (1989). Nucleotide sequence of rice dwarf virus genome segment 9. Journal of General Virology 70, 1297-1300.

Sano, T., Hataya, T., Terai, Y., and Shikata, E. (1989) Hop stunt viroid strains from dapple fruit disease of plum and peach in Japan. Journal of General Virology 70, 1311-1319.

1988年

Sano, T., Hataya, T., Shikata, E. (1988). Complete nucleotide sequence of a viroid isolated from Etrog citron, a new member of hop stunt viroid group. Nucleic Acids Research 16, 347-347.

1987年

Lee, S.Y., Uyeda, I., and Shikata, E. (1987). Characterization of RNA polymerase associated with rice ragged stunt virus. Intervirology 27, 189-195.

1986年

Sano, T., Hataya, T., Sasaki, A., and Shikata, E. (1986). Etrog citron is latently infected with hop stunt viroid-like RNA. Proceedings of the Japan Academy Series B-physical and Biologycal Sciences 62, 325-328.

Sano, T., Hataya, T., Terai, Y., and Shikata, E. (1986). Association of a viroid-like RNA from plum dapple disease occurring in Japan. Proceedings of the Japan Academy Series B-physical and Biologycal Sciences 62, 98-101.

Sano, T., Ohshima, K., Hataya, T., Uyeda, I., Shikata, E., Chou, T.G., Meshi, T., and Okada, Y. (1986). A viroid resembling hop stunt viroid in grapevines from Europe, the United-States and Japan. Journal of General Virology 67, 1673-1678.

1984年

Sano, T., Uyeda, I., Shikata, E., Ohno, T., and Okada, Y. (1984). Nucleotide sequence of cucumber pale fruit viroid: homology to hop stunt viroid. Nucleic acids research 12, 3427-3434.

Uyeda, I., and Shikata, E. (1984). Charakterization of RNAs synthesized by the viroid-associated transcriptase of rice dwarf virus in vitro. Virus Research 1, 527-532.